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Wednesday, February 22, 2012

Raman Spectroscopy in the Presence of Light Scattering: polymer model systems and applications toward biomedical and agricultural research

Kasimir Fajans Award in Chemistry / Analytical Analytical Seminar
1640 Chemistry
4pm - 5:30pm
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Speaker:   Matthew Schulmerich, University of Illinois
Abstract:   Raman spectroscopy has proven to be a non-destructive approach to obtaining chemically specific information from a wide variety of analytes. Conventional experimental configurations illuminate the sample and collect the backscattered Raman signal from the same points of illumination. However, by strategic illumination and collection of the Raman signal, even in the presence of light scattering, it is possible to tune the sampling depths to obtain signal from localized regions of interest. In this talk, a number of experimental examples will be presented illustrating applications for spatially offset Raman spectroscopy, dark field Raman spectroscopy, and transmission Raman spectroscopy. We illustrate the ability to gain spectral/chemical information with both spatial and depth resolution using polymer models, tissue phantoms as well as murine and human tissue. Considerations toward quantitative measurements will be illustrated using prostate tissue and soybeans.